Glutamate triggers elevation of intracellular Ca2+ concentration in neural precursor cells |
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Authors: | Naoko Yoshida Kiyoshi Yamada Shigeo Uchino Xiaofen Sun Takeshi Nakamura Yoshihisa Kudo Tatsuhiro Hisatsune Shuichi Kaminogawa |
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Affiliation: | (1) Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan;(2) Pharmaceuticals Discovery Laboratory, Yokohama Research Center, Mitsubishi Chemical Corporation, 1000 Kamoshida-cho, Aoba-ku, Yokohama, 227-8502, Japan;(3) Department of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0355, Japan;(4) Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan) |
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Abstract: | Both neurons and glial cells are derived from neuralprecursor cells in the ventricular zone during braindevelopment. The fate of the neural precursor cells isaffected by neurotransmitters such as glutamate. Inthis study, we examined glutamate-triggeredintracellular Ca2+ signaling in neural precursorcell lines by the calcium digital imaging method. Whenimmortalized primary-cultured neural precursor cellswere treated with glutamate, a subpopulation of thesecells showed an increase in intracellular Ca2+concentration. In an effort to determine the role ofthe glutamate-triggered intracellular Ca2+ signalin neural precursor cells, we tried to cultureimmortalized basal ganglial and hippocampal neuralprecursor cell lines in glutamate-free medium. Thehippocampal (MHP-2) cells became adapted to theglutamate-free medium, and when treated with glutamatethe adapted subline (MHP-2-E1) showed an increase inintracellular Ca2+ concentration. In contrast,the basal ganglial neural precursor cell lines failedto become adapted to the glutamate-free medium. Theseresults suggest that hippocampal and basal ganglialneural precursor cells differ in their cellularresponse to glutamate as an exogenous stimulus. |
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Keywords: | basal ganglia elevation of intracellular Ca2+ concentration glutamate hippocampus immortalized cell lines neural precursor cells |
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