Solvent-free enzymatic synthesis of feruloylated diacylglycerols and kinetic study |
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Authors: | Shangde Sun Liang Shan Yuanfa Liu Qingzhe Jin Yiping Song Xingguo Wang |
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Affiliation: | aState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu Province, PR China |
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Abstract: | The enzymatic esterification of glyceryl ferulate (FG) and oleic acid (OA) for feruloylated diacylglycerols (FDAG) synthesis in a solvent-free system was studied in this work. The reactions were catalyzed by different commercially available lipases, among which Novozym 435 was found to be the most active biocatalyst. The effects of glycerol in the reaction mixture and various synthesis parameters on yield of FDAG and the initial reaction rate were studied. The optimum synthesis conditions were as follows: temperature, 65 °C; enzyme load, 7.5%; substrate ratio, 7.5:1 (OA/(FG + glycerol), w/w); and reaction time, 12 h. Under the optimum conditions, the conversion of FG and yield of FDAG reached 98.0 ± 1.0% and 82.6 ± 2.2%, respectively. A linear relationship was established between the initial reaction rate and enzyme load up to 10%, which demonstrated that the influence of external mass transfer limitations on the reaction could be eliminated. The relationship between initial reaction rate and temperature was also established, based on the Arrhenius law. Novozym 435 in the present work can be used 18 times under the optimum conditions without essential losses in activity. The reaction kinetics agrees with the Ping-Pong Bi-Bi mechanism characterized by Vm and K′m values of 5.26 × 10−4 mol/(L min) and 0.26 mol/L, respectively. |
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Keywords: | Feruloylated diacylglycerols (FDAG) Glyceryl ferulate (FG) Oleic acid (OA) Lipase-catalyzed esterification Kinetic model |
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