| 海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析 |
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| 引用本文： | 车海彦,罗大全,杨旭光,符瑞益,叶莎冰.海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析[J].Virologica Sinica,2005,20(5):543-545. |
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| 作者姓名： | 车海彦 罗大全 杨旭光 符瑞益 叶莎冰 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所 海南儋州571737
(车海彦，罗大全，杨旭光，符瑞益)，中国热带农业科学院环境与植物保护研究所 海南儋州571737(叶莎冰) |
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| 基金项目： | 中国热带农业科学院科技基金(Rky0303) |
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| 摘 要： | 粉虱传双生病毒(Whitefly-transmitted gemini-virus,WTGV)是一类广泛发生在热带、亚热带地区植物上的具有孪生颗粒形态的单链DNA病毒,在分类上属双生病毒科(Geminiviridae)的菜豆金色花叶病毒属(Begomovirus),该属的大多数病毒都由2个组分(DNA-A和DNA-B)组成,为两条闭合环状ssDNA分子,长度相似,每条为2.5-2.8kb,少数病毒为单组分,仅有DNA-A组分1]。我国自1983年报道发现双生病毒以来,在云南、广西、广东和海南等省区的已相继发现多种双生病毒2~6],表明这类病毒在我国的危害有蔓延和加重的趋势。本文对从海南番木瓜(Carica pap…
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| 关 键 词： | 粉虱传双生病毒 海南 PCR 序列分析 |
| 收稿时间： | 2005-03-17 |
| 修稿时间： | 2005-06-13 |
Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan |
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| CHE Hai-yan, LUO Da-quan, YANG Xu-guang, FU Rui-yi, YE Sha-bing.Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan[J].中国病毒学(英文版),2005,20(5):543-545. |
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| Authors: | CHE Hai-yan LUO Da-quan YANG Xu-guang FU Rui-yi YE Sha-bing |
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| Institution: | Enviromental and Plant Protection Institute , Chinese Academy of Tropical Agricultural Sciences ,Danzhou 571737, China |
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| Abstract: | Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses(WTGV),PCR product of expected size was amplified from six samples.The specific fragments were cloned and sequenced,and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST.Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity.Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG,P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch. |
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| Keywords: | Whitefly-transmitted geminiviruses PCR Sequence analysis |
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