p150Sa12在人卵巢癌细胞株中促进MX1基因的表达

目的：研究p150Sa12在人卵巢癌细胞株中对MX1基因表达的影响。方法：将MX1基因的启动子克隆至带有Luciferase报告基因的pGL3-basic载体中，将其与p150Sa12表达载体用PEI介导的转染方法共转染至卵巢癌细胞株SKOV3，用双荧光素酶检测系统检测MX1启动子活性变化；将p150Sa12表达载体转染至卵巢癌细胞株ES-2，用western blot检测细胞中MX1基因的表达变化。结果：双荧光素酶检测系统检测MX1启动子活性被p150Sa12上调，westemblot检测转染p150Sa12后细胞中MX1表达量增加。结论：D150Sa12在人卵巢癌细胞株申促进MX1基因的表达。

Induction of MX1 Expression by p150Sa12 in Human Ovarian Cancer Cell Line

Objective： To investigate the effect ofp150Sa12 on MX 1 gene expression of human ovarian cancer cell line. Methods： Promoter of MX1 gene was cloned into pGL3-basic with luciferase reporter gene, and co-transfected with p 150Sa12 expression plasmid into SKOV3 cell line. The promoter activity was detected by using dual-luciferase reporter gene detection method. The MX1 expression in ES-2 cells was assayed after transfection with p150Sa12 expression plasmid by westem blot. Results： The activity of MX1 promoter was up-regulated by plSOSal2 in SKOV3 cell line, and the western blot result showed increased expression of MX1 gene of ES-2 cell line transfected with pl50Sal2 expression plasmid. Conclusions： MX1 expression was induced by pl50Sal2 in human ovarian cancer cell line.