Ghrelin对缺氧复氧状态下脂肪间充质干细胞保护作用的研究

目的：探讨缺氧复氧损伤环境下Ghrelin对脂肪来源的间充质干细胞（AD-MSCs）的保护作用,以寻求AD-MSCs心肌内移植的有利因素。方法：采用胶原酶消化法分离小鼠AD-MSCs,流式细胞术鉴定其标志。建立缺氧/复氧细胞模型,分3组：①对照组;②缺氧/复氧组（H/R）;③H/R＋Ghrelin（浓度分别为10-9、10-8、10-7mol/L）干预组。MTT法测定各组细胞增殖,TUNEL法检测细胞凋亡。结果：流式细胞术结果显示AD-MSCs CD44及CD90阳性,CD34、CD45阴性。AD-MSCs MTT分析显示在缺氧环境中,Ghrelin相比于单纯H/R组能够显著促进AD-MSCs的存活与增殖,并抑制其凋亡（P〈0.05）。结论：Ghrelin可以明显提高缺氧复氧环境下AD-MSCs的生存与增殖,抑制缺氧诱导的凋亡发生,有望为心肌梗死的干细胞移植治疗创造新的有利因素。

Protection of Ghrelin on Adipose-derived Mesenchymal Stem Cells under Hypoxia-reoxygenation Injury

Objective：To investigate the effect of Ghrelin on adipose tissue derived mesenchymal stem cells（AD-MSCs） under hypoxia-reoxygenation（H/R） injury,and to explore the protective factors for myocardial cell transplantation.Methods：AD-MSCs were isolated from Kunming mice and characterized by flowcytometry.AD-MSCs were divided into（1） Normal control（2） Hypoxia＋ Reoxygenation（H/R）（3） H/R＋Ghrelin（10-9,10-8,10-7 mol/L,respectively）.Cell survival and proliferation were assessed by MTT assays.Apoptosis of AD-MSCs was detected by TUNEL strain.Results：AD-MSCs were positive to the CD44 and CD90.The pretreatment of Ghrelin improved the survival and proliferation of AD-MSCs under H/R condition compared with that of H/R group（P0.05）.TUNEL showed Ghrelin inhibited the apoptosis of AD-MSCs after H/R injury（P0.05）.Conclusions：Ghrelin has beneficial effects on the survival and proliferation of AD-MSCs under H/R environment and might constitute a hopeful target in stem cell transplantation for ischemic cardiovascular disease.