基于NR2B基因探讨疏肝与补肾法对吗啡精神依赖的调节作用及机制

目的：探讨补肾法与舒肝法对大鼠吗啡精神依赖的调节作用及机制。方法：使用盐酸吗啡建立大鼠精神依赖模型（conditioned place preference，CPP），采用Obersiver5．0行为学软件分析大鼠的CPP效应，并利用RealtimePCR方法测定伏核和前额叶皮质内NR2B亚基的mRNA含量。结果：与对照组相比，模型组大鼠在白侧累积停留时间极显著长于对照组（P〈0．01），同时伏核与前额叶皮质中NR2B的mRNA水平也显著升高（P〈0．01和P〈0．05）；与模型组相比，补肾组和舒肝组大鼠在白侧的累积停留时间显著下调（P〈0．05），疏肝组NR2B的mRNA也显著下降（P〈0．05），而补肾组变化不显著。结论：①补肾法与舒肝法对吗啡引起的精神依赖均有调节作用。②舒肝法的作用机制可能在于通过影响伏核和前额叶皮质中NR2B亚基的mRNA表达进一步调节精神依赖的相关神经通路。③补肾法的作用机制与NR2B亚基的mRNA表达关系不密切，其调节的具体机制尚有待研究。

Discussion of Liver-Smoothing and Kidney-Nourishing Therapy for Psychological Dependence and Its Mechanism Based on NR2B

Objective： To analyze the effect and mechanism of liver-smoothing therapy and kidney-nourishing therapy to morphine-induced conditioned place preference. Methods： Morphine was injected to set up the conditioned place preference model in rats, Obersiver 5.0 Behavioral Software was used to analyze the time of staying in morphine-paired compartment, and real time PCR was used to detect the mRNA of NR2B. Result： The morphine model group, compared to the control group, the time of staying in morphine-paired compartment were significantly up regulated （P〈0.01）, and the mRNA of NR2B in nucleus accumbens and prefrontal cortex were up regulated as well （P 〈 0.01 and P 〈 0.05）. Compared to the morphine group, in the liver-smoothing therapy group, both the time of staying in morphine-paired compartment and mRNA were significantly reduced （P 〈0.05）, but in the kidney-nourishing therapy, only the time of staying in morphine-paired compartment were significantly reduced （P 〈0.01）. Conclusion： （1）The morphine-induced conditioned place preference can be regulated by both liver-smoothing therapy and kidney-nourishing therapy. （2）The regulating mechanism of liver-smoothing therapy to morphine-induced conditioned place preference may be related to the level of mRNA of NR2B in nucleus accumbens and prefrontal cortex. （3）The regulating mechanism of kidney-nourishing therapy to morphine-induced conditioned place preference should not be related to the level ofmRNA of NR2B in nucleus accumbens and prefrontal cortex.