| 一种用于CYP3A5基因分型的电化学传感器阵列设计 |
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| 引用本文： | 张海燕,王捷,陈钰,吴小丽,刘仲明. 一种用于CYP3A5基因分型的电化学传感器阵列设计[J]. 生物磁学, 2011, 0(15): 2967-2969 |
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| 作者姓名： | 张海燕 王捷 陈钰 吴小丽 刘仲明 |
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| 作者单位： | [1]广州军区广州总医院医学实验科,广东广州510010 [2]中国科学院传感技术联合国家重点实验室,上海200050 |
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| 基金项目： | 广东省自然科学基金博士启动资助项目（10451001002004935）传感技术联合国家重点实验室基金资助项目（Skt1003） |
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| 摘 要： | 目的：设计一种用于检测CYP3A5基因分型的电化学传感器阵列及其不同基因型的判别方法。方法：设计的电化学基体由印刷电路板（PCB）组成，该电路板包含一组金电极。每个金电极表面修饰有包含单链捕获探针的自组装单分子膜。设计中使用二茂铁做为电活性指示剂，基因分型检测是通过两种不同电势的二茂铁衍生物分别标记等位基因特异性信号探针来实现。结果：该设计能构建一种快速准确、操作简便的DNA电化学传感器阵列检测系统。结论：本文设计为使用电化学方法检测基因分型提供了一种新方法和新技术。
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| 关 键 词： | 传感器 基因分型 检测 |
A Design of Electrochemical Sensor for Detection of CYP3A5 Genotyping |
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| ZHANG Hai-yan,WANG Jie,CHEN Yu,WU Xiao-li,LIU Zhong-ming. A Design of Electrochemical Sensor for Detection of CYP3A5 Genotyping[J]. Biomagnetism, 2011, 0(15): 2967-2969 |
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| Authors: | ZHANG Hai-yan WANG Jie CHEN Yu WU Xiao-li LIU Zhong-ming |
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| Affiliation: | 1 Department of Medical Research, the General Hospital of Guangzhou Command, PLA, Guangzhou, 510010, China; 2 State Key Laboratory of Transducer Technology, Chinese Academy of Science, Shanghai,200050s) |
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| Abstract: | Objective： To design a new type of array that utilizes electrochemical reactions and a novel method of simultaneously detection of CYP3A5 genotypings. Methods： The array is composed of a printed circuit board （PCB） consisting of an array of gold electrodes. Each electrode is modified with a self-assembled monolayer that includes oligonucleotide capture probes. Using ferrocene as the electro-active indicator, genotyping of polymorphisms uses allele-specific signal probes containing ferrocene labels with distinguishable redox potentials. Results： The design of electrochemical sensor array is accurate and convenient for detection DNA. Conclusion： It supplies a promising new mean/technique that utilizes electrochemical reactions for genotyping. |
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| Keywords: | Sensor Genotyping Detection |
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