| 大剂量HBsAg对HBV转基因小鼠T细胞免疫调节的研究 |
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| 引用本文： | 刘博,苏文静,马力,潘蕾,连建奇,黄长形,白雪帆. 大剂量HBsAg对HBV转基因小鼠T细胞免疫调节的研究[J]. 生物磁学, 2011, 0(5): 812-814 |
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| 作者姓名： | 刘博 苏文静 马力 潘蕾 连建奇 黄长形 白雪帆 |
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| 作者单位： | 第四军医大学唐都医院全军感染病诊疗中心,陕西西安710038 |
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| 基金项目： | 国家科技重大专项资助项目（2008ZX10204） |
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| 摘 要： | 目的：研究大剂量HBsAg对HBV转基因小鼠其T细胞免疫效果的影响。方法：用大剂量血源性HBsAg免疫HBV转基因小鼠,采用ELISA方法观察转基因小鼠所诱生的HBsAg特异性Th1类细胞因子的水平,ELISPOT方法检测不同免疫方案对小鼠HBsAg特异性分泌IFN-γT细胞数量的影响,同时检测对小鼠淋巴细胞增殖的影响。结果：HBsAg组免疫后脾细胞产生的Th1类细胞因子（IFN-γ、IL-2）、HBsAg特异性分泌IFN-γT细胞及T细胞增殖水平较对照组显著增加（P〈0.05）。结论：大剂量的HBs-Ag可以诱导乙肝转基因小鼠产生高水平Th1类细胞因子并打破免疫耐受。
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| 关 键 词： | 肝炎病毒 乙型 HBsAg 转基因小鼠 免疫耐受 |
Study on T Cellular Immune Response to Large-dose HBsAg in Transgenic Mice |
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| LIU Bo,SU Wen-jing,MA Li,PAN Lei,LIAN Jian-qi,HUANG Chang-xing,BAI Xue-fan. Study on T Cellular Immune Response to Large-dose HBsAg in Transgenic Mice[J]. Biomagnetism, 2011, 0(5): 812-814 |
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| Authors: | LIU Bo SU Wen-jing MA Li PAN Lei LIAN Jian-qi HUANG Chang-xing BAI Xue-fan |
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| Affiliation: | △(Infectious Diseases,Tangdu hospital,Fourth Military Medical University,Xi＇an,Shanxi,710038,China) |
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| Abstract: | Objective：To investigate the effect of HBsAg on T cellular immune responses in HBV transgenic mice.Methods：HBV transgenic mice were immunized by HBsAg and Sodium Chloride.The Th1 cytokines（IFN-γ,IL-2） were detected by ELISA and the number of IFN-secreting T cells was examined by ELISPOT.The proliferation of spleen cells was measured by MTT.Rsults：Compared with control group,the IL-2,IFN-γ and HBs-specific IFN-secreting T cell of HBsAg group increased,and strong lymphocyte proliferation reaction was induced（p0.05）.Conclusion：HBsAg could increase Th1 cytokines in transgenic mice and break the immune tolerance to antigen in HBV transgenic mice. |
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| Keywords: | Hepatitis B virus HBsAg Transgenic mice Immune tolerance |
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