| 耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价 |
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| 引用本文： | 谌海燕,侯丽,陈信义.耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价[J].生物磁学,2011(5):841-843. |
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| 作者姓名： | 谌海燕 侯丽 陈信义 |
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| 作者单位： | [1]北京中医药大学东直门医院,北京100700 [2]北京市与教育部重点实验室（中医内科学）,北京100700 |
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| 基金项目： | 北京中医药大学自主创新课题（2009TYBZZ-XS057） |
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| 摘 要： | 目的：建立耐三苯氧胺（TAM）人乳腺癌的裸鼠移植瘤模型,为研究和治疗乳腺癌对TAM耐药提供研究工具。方法：采用雌激素受体阳性,对TAM耐药的人乳腺癌细胞系LCC2,接种于BALB/c裸鼠皮下,观察肿瘤生长趋势,用免疫组化方法进行鉴定。结果：在接种细胞数大于5×106/只时,Matrigel能够显著促进移植瘤的生长。肿瘤组织病理学检测证实为浸润性导管癌,且Pgp和Her-2为阳性表达。结论：该方法建立的耐TAM人乳腺癌移植瘤模型,周期短,成瘤率高,保留了与细胞系相同的肿瘤生物学特征。
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| 关 键 词： | 乳腺癌 移植瘤 三苯氧胺 |
Establishment of xenograft model in nude mice of tamoxifen-resistanthuman breast cancer |
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| CHEN Hai-yan,HOU Li,CHEN Xin-yi.Establishment of xenograft model in nude mice of tamoxifen-resistanthuman breast cancer[J].Biomagnetism,2011(5):841-843. |
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| Authors: | CHEN Hai-yan HOU Li CHEN Xin-yi |
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| Institution: | 1Dongzhimeng Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;2The Key Laboratory of Beijing (Traditional Chinses Physician),Beijing 100700,China) |
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| Abstract: | Objective：This study aims to establish the xenograft model of human breast cancer resistant to tamoxifen（TAM）so as to provide a research tool for the study and treatment of breast cancer resistant to TAM.Methods：This study employs the method of inoculating the human breast cancer cells LCC2 which is estrogen receptor positive and resistant to TAM into BALB/c nude mice.Then it observes the trends of tumor growth,and identifies by immunohistochemistry methods.Results：When the number of inoculation cells is more than 5×106,Matrigel can significantly promote tumor growth.Pathology confirms that the xenograft is invasive ductal carcinoma,and Pgp,Her-2 are positively expressed.Conclusions：The method of establishing the xenograft model of human breast cancer resistant to TAM has short cycle,high rate of tumor formation,and retains the same characteristics of tumor biology with cell lines. |
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| Keywords: | Breast Cancer Xenograft Tamoxifen |
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