抗中性粒细胞胞浆抗体、抗核抗体联合检测对类风湿关节炎的诊断价值

目的：探讨抗中性粒细胞胞浆抗体（ANCA）与抗核抗体（ANA）联合检测对类风湿关节炎的临床意义。方法：采用IIF法对82例RA患者（RA组）、74例非RA自身免疫疾病患者（非RA组）和52例健康体检者（正常对照组）的血清ANCA和ANA谱进行了检测分析，并用ELISA法进行抗丝氨酸蛋白酶3（PR3）、抗髓过氧化物酶（MPO）、ANA谱的定量检测。结果：RA组82例患者中，64例ANCA阳性，阳性率为78．08％，其中核周型（PANCA）37例，阳性率为45．1％，胞浆型（CANCA）27例，阳性率为32．9％；非RA组74例患者中有7例ANCA阳性率分别为9．4％；正常对照组50例中没有一例ANCA阳性。利用Elisa法对患者血清进行检测，分别能够特异的检测到PR3、MPO、抗双链DNA抗体（抗ds—DNA抗体）、抗SS—A等抗体、抗ss—A抗体、抗PM—SCL抗体的存在。结论：联合ANCA、ANA检测有助于提高类风湿关节炎的诊断。

Diagnostic Value of ANCA Combining with ANA Tests in Patients with Rheumatoid Diseases

Objective： To study the clinical significance of antineutrophil cytoplasmic antibody （ANCA） combining with antinuclear antibody （ANA） tests in patients with reheumatiod（RA） diseases. Methods： The spectrums of ANCA and ANA in RA group （n=82）,non-RA group （n=74） and healthy control group （n=52） were tested by lndirect-lmmunen-Flurocence methods;Anti-serine proteinase 3, anti-MPO and ANA spectrum were detected by ELISA. Results： In RA group, there were 64 patients with ANCA-positive, the positive rate was 78.08%;in non-RA group, 7 patients with ANCA-positive, the positive rate, 9.4%; there were no ANCA-positive patients in the healthy control group. Conclusion： The joint detection of ANA and ANCA is helpful for the diagnosis of rheumatoid diseases.