炭疽受体CMG2-Fc融合蛋白在CHO细胞中的表达、纯化与鉴定

目的：构建炭疽受体CMG2和人IgGl Fc片段融合基因载体，转染CHO细胞并通过毒素中和试验检测CMG2-Fc拮抗炭疽毒素（PA＋LF）的能力。方法-将含有CMG2胞外区1-217AA片度基因和人IgGl的Fc片段基因共同连接入pcDNA3．1载体转染CHO细胞并筛选高表达CMG2-Fc的CHO细胞系，通过小鼠RAW264．7巨噬细胞保护试验检测CMG2-Fc拮抗炭疽毒素的能力。结果：获得了表达CMG2-Fc的细胞株，毒素中和实验显示该蛋白可以有效抑制炭疽毒素引起的细胞损伤。结论：CMG2-Fc能够保护小鼠巨噬细胞免受炭疽毒素攻击，提示其可以作为抗毒素治疗炭疽感染。

Expression,Purification and Identification of Anthrax Receptor CMG2-Fc Fusion Protein in CHO Cell

Objective： To construct fusion gene vector of anthrax receptor CMG2 and human IgG1 Fc fragent, transfect CHO cell and to testify the inhibit ability of CMG2-Fc on anthrax toxin （PA and LF） by toxin neutralization assay. Methods： An expression vector including CMG2 （1-225AA） gene and Fc fi＇agment of human immunoglobulin G （IgG） were constructed, and CHO cell line with higher CMG2-Fc expression were got. The effect of CMG2-Fc on inhibiting anthrax toxin was detected by mouse maerophage protection assay. Results： The CHO cell line expressing CMG2-Fc protein were got, and toxin neutralization assay showed that CMG2-Fc could protect mouse RAW264.7 macrophage cell against anthrax toxins. Conclusion： CMG2-Fc can protect mouse maerophage against anthrax toxin challenge, which indicated that it maybe used as drugs against anthrax in future.