糖尿病大鼠膀胱和骶髓背根神经节中NGF的表达与尿流动力学改变的研究

目的：探讨糖尿病大鼠膀胱与骶髓背根神经节（DRG）中神经生长因子（NGF）的表达与尿流动力学改变。方法：建立糖尿病大鼠模型10只,对照组10只,应用酶联免疫吸附试验（ELISA）法,分别检测大鼠膀胱组织及骶髓DRG中NGF的变化情况,结合代谢笼及尿流动力学改变,探讨糖尿病膀胱病变的可能发病机制。结果：造模12周后,糖尿病大鼠膀胱容量较正常对照组明显增大（1.47±0.28vs0.71±0.12,p〈0.05）,残余尿量明显增多（0.52±0.18vs0.07±0.08,p〈0.01）,排尿效率明显下降,膀胱及骶髓DRG中NGF表达水平明显降低。结论：NGF在糖尿病大鼠膀胱和骶髓背根神经节中低表达,在糖尿病膀胱病变中发挥着重要作用。

Expression of NGF in lumbar dorsal root ganglia and bladder of diabetic rats and urodynamic changes

Objective：To evaluate the expression of nerve growth factor（NGF） in the bladder and sacral cord dorsal root ganglia（DRG） and the bladder function 8 weeks after diabetes induction and investigate the pathogenesis of diabetic cystopathy.Methods：Twenty Sprague-Dawley rats were divided into two groups：control（n=10）,streptozotocin-induced diabetic group（n=10）.Eight weeks later,the bladders and sacral cord DRG were dissected.We measured the expression of NGF in the bladders and sacral cord DRG using ELISA,evaluated the bladder function by metabolic cage study and cystometry.Results：We found that the streptozotocin-induced diabetic rats showed impaired bladder function characterized by increased bladder capacity,decreased bladder contractility（voiding efficiency）,and an increase in residual urine.We also found a significantly reduced expression of NGF in the bladders sacral cord DRG from the diabetic group compared with the control.Conclusions：Our findings indicated that the decrease in NGF may be a contributory factor in diabetic cystopathy.