评价空肠弯曲菌外膜蛋白PEB1介导的重组BCG与上皮细胞的结合能力

目的：评价PEBl介导的屋尘螨抗原（Derp2）重纽BCG疫苗（PEBI-Derp2．rBCG）与人上皮细胞的结合能力。方法：采用体外细胞培养的方法，分别将普通BCG、胞壁型Derp2-rBCG和胞壁型融合蛋白PEBl-Derp2-rBCG与HeLa细胞及人类肠粘膜上皮细胞（HIEC）进行共孵育，利用HE和抗酸染色法对各组细胞与疫苗的黏附结果进行染色，光学显微镜下计数各组的黏附率，并进行比较；对以上各组分别加入PEBl蛋白，进行黏附阻断，观察对结合能力的影响。结果：孵育24小时后，无论HeLa细胞还是H匝CPEBl-Derp2．rBCG组较普通BCG组和Derp2-rBCG组的黏附率明显提高，差异有显著性（P〈0．05）；PEBl蛋白的加入对PEBl．Derp2-rBCG的黏附功能有明显抑制作用（P〈O．05）；但是，Derp2-rBCG组与普通BCG组比较没有明显差异（P〉o．05），PEBl蛋白的加入对二者的黏附亦无影响（P〉0．05）。结论：PEBl具有介导增强PEBl-Derp2-rBCG与上皮细胞黏附的能力。

Evaluation of Combination Ability Between Recombinant BCG Mediated by Membrane Protein PEB 1 of C.Jeiuni and Epithelium Cells

To investigate the combination ability of the PEB1-Der p2- rBCG and epithelium cells. Methods： The method of cell culture in vitro was used to culture the bacterial strains of common BCG, membrane form Der p2-rBCG and membrane form PEB1-Der p2- rBCG with HeLa and human intestinal epithelial cells （HIEC）, HE staining and acid-fast staining were used and the adhesion of cells was detected at light microscopy; purified PEBlwas added into each group to adhesion blocking,and the influence of combining ability was observed. Results： After incubated for 24 h, no matter HeLa cells or HIEC, adhesion rate of PEB 1-Derp2- rBCG was obviously improved than common BCG and Der p2-rBCG, and the differences were statistically significant （P 〈 0.05）; The differences between PEB 1-Der p2- rBCG performed in the absence and presence of PEB 1 protein were significant （P 〈 0.05）; However, the Der p2-rBCG bacteria group was not significantly different from common BCG group （P 〉 0.05） and no adhesion blocking effects in the two group which added PEB1 protein （P 〉 0.05）. Conclusion： The PEB1 increased the adhesion ability which mediated PEB1- Der p2- rBCG and epithelium cells.