钙网质蛋白在多囊卵巢综合征大鼠子宫内膜的表达及意义

目的：研究钙网质蛋白（CRT）在PCOS大鼠子宫内膜中的表达及生物学意义。方法：60只雌性SD大鼠随机分为PCOS组和对照组，每组各30只。给模型组24日龄大鼠皮下埋植左旋甲基炔诺酮硅胶棒3mm／只，3d后BID皮下注射人绒毛膜促性腺激素1．5IU。给对照组皮下注射等体积生理盐水。注射9d后观察大鼠卵巢形态学（HE染色），化学发光法测定性激素水平。结果：模型组大鼠卵巢重量和体积均显著高于对照组（P〈0．01）。模型组大鼠卵巢出现类多囊卵巢综合征的改变。模型组卵巢各级发育期卵泡及黄体少见，卵泡多呈囊性扩张。模型组大鼠血清孕激素、睾酮、空腹胰岛素、空腹血糖水平均显著高于对照组（P〈0．05）；卵泡刺激素（FSH）水平显著低于对照组（P〈0．05）。LH／FSH比值显著高于对照组（P〈0．05）。采用免疫组织化学方法及灰度值测定，定量分析CRT在PCOS组和对照组的子宫内膜中表达。CRT在两组中的子宫内膜中均有表达。PCOS组子宫内膜上皮CRT表达显著低于对照组（P〈0．01）。结论：避孕硅胶棒联合hCG诱导SD大鼠多囊卵巢综合征模型是较好的PCOS模型。CRT与PCOS的发病密切相关．

Expression and Significance of Calreticulin in Endometrium of Rats with Polycystic Ovarian Syndrome

Objective： To investigate the expression and biological significance of apoptosis regulatory CRT in the follicles and endometrium ofpolycystic ovarian syndrome （PCOS） in rat model. Methods： Sixty rats were randomly divided into two groups： PCOS group （n=30） and control group （n= 30）. The rats in the experimental group were given subcutaneous implanting of levonorgestrel silica gel staffand injected 115 I U hCG twice daily for 9 days from the 4th day. The rats in the control group were injected with normal saline at the same time. Ovarian morphologic changes, sex hormone levels were detected. Results： The weight and volume of ovarian in the model rats were higher than that in the control rats＇ （P〈0.01）. The rats in model group had similar pathological appearance to polycystic ovary. The multiple follicular cysts, and the number ofthecacells and interstitial cells increased in the ovaries of modeling group showed. There was less developed follicles and corpus lutea. The progesterone, testosterone, luteinizing hormone, fasting insulin, glucose the LH /FSH ratio were significantly higher in experimental group than those in control group, so as. There was the expression of apoptosis regulatory protein CRT. The CRT protein expressed in endometrium in PCOS group and control group. The expression of CRT of endometrial in PCOS rats were significantly lower （P 〈 0.01） than those in control group. Conclusion： The rat model induced by levonorgestrel with hCG was proved to be an ideal PCOS animal model. CRT may be closely correlated with the pathogenesis of PCOS.