RGD肽介导的MR分子探针在体外结直肠癌细胞的显像及对其生物学行为的影响

目的：探讨RGD肽介导的MR分子探针在体外结直肠癌细胞的MRJ显像及对其生物学行为的影响。方法：利用纳米技术构建靶向RGD荧光纳米MR、探针，利用荧光倒置相、差显微镜观察该探针与LOVO细胞结合情况；体外磁共振成像（MRI）显像；利用细胞克隆实验测其增殖活性；流式细胞术检测其细胞周期、凋亡。结果：荧光相差显微镜示该RGD肽介导的MR分子探针能特异性与LOVO细胞结合；体外MRI成像示靶向RGD组T1信号强度高于非靶向组及对照组（P〈0．05）；该探针作用24h后的LOVO细胞增殖活性降低，细胞分裂周期发生变化，阻滞在S＋G2M期的细胞比例上升，细胞凋亡率与其他两组相比有显著增加（P〈0．05）。结论：该RGD肽介导的MR分子探针能与结直肠癌LOVO细胞靶向结合，能增强MR1的显像效果，并对肿瘤细胞具有一定的抑制作用．

The Vitro Imaging of RGD Peptide-Mediated MR Molecular Probes and Its Influence in Colorectal Cancer Cells

Objective： To investigate the effect of the RGD peptide-mediated MR molecular probes on the MRI application of LOVO cells in vitro and the biological behavior. Methods： The nano-technology constructed RGD peptide-mediated MR molecular probes were used. The binging situation was detected by the fluorescence phase contrast microscope, MRI test T~ signal intensity. The cell colon and Flow cytometry were used to analyze the proliferative situation. Result： The RGD peptide-mediated MR molecular probes can binding with the LOVO cell specificity. The signal intensity of targeted probe group was higher than that in others two groups（P〈0.05）. The cell proliferation activity of targeted probe group decreased. RGD peptide-mediated MR molecular probes can block G2＋S period of LOVO cell and increased the apoptosis rate （P〈0.05）. Conclusions： The RGD peptide-mediated MR molecular probes can specifically adsorbed with colorectal cancer LOVO cells, and it can target enhanced the MRI imaging results, and may have some inhibited effect on tumor cells.