抑癌基因p16对人肝癌细胞生长抑制的机制研究

目的：探讨抑癌基因p16对肝癌细胞生长的抑制作用及其机制。方法：将p16cDNA亚克隆至pcDNA3．1真核表达载体上，并经脂质体介导转染至人肝癌细胞株SMMC-7721：用MTT法和Western blot分析转染细胞的生长情况。结果：成功构建重组表达质粒pcDNA3．1-p16，转染pcDNA3．1-p16的SMMC-7721细胞生长速度受到明显抑制；转染后有外源p16蛋白的表达，且伴随Bax上调，Bcl-2和cIAP2的下调。结论：重组pcDNA3．1-p16质粒能在人肝癌细胞SMMC-7721内表达，且能抑制SMMC-7721的生长，其机理与诱导肿瘤细胞凋亡相关。

The Growth inhibitory Effects by Transfection of Anti-Oncogene P 16 on Human Hepatocarcinoma Cell Line

Objective： To investigate the inhibitory effect of tumor suppressor gene p 16 on the growth of hepatocarcinoma cell. Methods： p16 eDNA was subcloned into pcDNA3.1 eucaryotic expressing vector; then the recombinant pcDNA3.1-pl6 plasmid was transfected into human hepatocarcinoma cell line SMMC-7721 with liposome. MTT method and Western blot were employed to investigate cell growth. Results： The recombinant plasmid pcDNA3.1-p16 was constructed successfully; the growth of hepatocarcinoma cell line SMMC-7721 was obviously inhibited after transfection; the exogenous p 16 protein was expressed, with the up-regulation of Bax as well as down-regulation of Bcl-2 and cIAP2. Conclusion： The recombinant plasmid pcDNA3.1-p16 is able to express p16 protein in human hepatocarcinoma cell line SMMC-7721 and suppress the cancer cell growth, which is associated with apoptosis.