胰蛋白酶诱导人脐静脉内皮细胞分泌白细胞介素-8

目的：研究胰蛋白酶对IL-8释放的影响。方法：分离、培养人脐静脉内皮细胞（human umbilical vein endothelialcells，HU-VECs）、倒置显微镜观察形态变化，流式细胞术检测内皮细胞标志和蛋白酶活化受体．2（proteinase．activatedreceptor．2，PAR-2）表达，ELISA检测HUVECs培养上清中IL-8水平。结果：HUVECs表达内皮细胞标志和PAR-2。刺激16h，1g／ml胰蛋白酶和100MPAR-2激活肽组HUVECs单层均匀性降低。胰蛋白酶能够显著刺激HUVECs释放IL-8，PAR-2激活肽也诱导IL-8水平升高。蛋白酶抑制剂和PAR-2抑制肽均能够显著抑制胰蛋白酶诱导的IL-8释放。PAR-2激活肽和胰蛋白酶诱导升高的IL-8水平之间成正相关性。结论：胰蛋白酶很可能通过PAR-2激活促进血管内皮细胞释放IL-8。

Induction of Interleukin-8 Release from Human Umbilical Vein Endothelial Cells by Trypsin

Objective： To investigate the effects of trypsin on interleukin-8 release. Methods： Human umbilical vein endothelial cells （HUVECs） were solated, cultured, and the monolayers in apperance were observed by inverted microscope. Expression of endothelial markers and proteinase-activated receptor-2（PAR-2） was detected by flow cytometry, and the IL-8 levels in culture supemants from HUVECs by ELISA. Results： There was the expression of endothelial markers and proteinase-activated receptor-2 （PAR-2）. The uniformity ofmonolayers decreased in HUVECs treated with 1 g/ml trypsin and 100 M PAR-2 peptide. Trypsin was able to up-regulate IL-8 release. A PAR-2 agonist peptide also significantly enhanced IL-8 release. Trypsin-induced IL-8 release was inhibited by proteinase inhibitors, and an inhibitor peptide. There was positive correlation between the IL-8 levels induced by trypsin and PAR-2 agonist peptide. Conclusion： Trypsin promotes IL-8 release most likely through PAR-2 activation.