| 单克隆抗体MGd1对胃癌SGC-7901细胞增殖和凋亡的影响 |
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| 引用本文： | 安艳新,任贵,田启飞,赵青川.单克隆抗体MGd1对胃癌SGC-7901细胞增殖和凋亡的影响[J].生物磁学,2011(8):1409-1412. |
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| 作者姓名： | 安艳新 任贵 田启飞 赵青川 |
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| 作者单位： | 第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032 |
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| 基金项目： | 国家重点基础发展计划项目（973计划）（No.2009CB521705）; 国家自然科学基金（No.30971337） |
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| 摘 要： | 目的：研究不同浓度的MGd1对体外培养胃癌细胞SGC-7901增殖及凋亡的影响。方法：采用MTT法测定不同浓度MGd1对SGC-7901生长抑制作用;流式细胞术（FCM）进行细胞凋亡分析。激光共聚焦显微镜观察MGd1抗原（MGd1-Ag）的亚细胞定位。结果：MTT结果显示不同浓度的MGd1均对SGC-7901细胞产生明显的抑制效应（P=0.02）;流式细胞术分析发现MGd1可诱导SGC-7901发生凋亡并呈浓度和时间依赖性（P〈0.01）;共聚焦显微镜结果显示MGd1-Ag主要定位于细胞膜上。结论：以上结果证实胃癌特异性单抗MGd1可抑制SGC-7901的增殖并促进凋亡发生。它可能通过与细胞膜上抗原特异性结合,影响下游信号传导,从而发挥抑制效应。
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| 关 键 词： | 单克隆抗体 MGd1 增殖 凋亡 |
Effect of Monoclonal Antibody MGd1 on Proliferation and Apoptosis of Gastric Cancer Cell SGC-7901 |
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| AN Yan-xin,REN Gui,TIAN Qi-fei,ZHAO Qing-chuan.Effect of Monoclonal Antibody MGd1 on Proliferation and Apoptosis of Gastric Cancer Cell SGC-7901[J].Biomagnetism,2011(8):1409-1412. |
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| Authors: | AN Yan-xin REN Gui TIAN Qi-fei ZHAO Qing-chuan |
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| Institution: | (State Key Laboratory of Cancer Biology ＆ Xijing Hospital of Digestive Diseases, Fourth Military Medical University,Xi＇an 710032,P.R.China) |
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| Abstract: | Objective： To investigate the effects of MGd1 with different concentration on the cell proliferation and apoptosis of gastric cancer cell line SGC-7901.Methods： Cell proliferation was detected by MTT assay and cell apoptosis was analyzed by flow cytometry.Subcellular localization of MGb2-Ag in SGC-7901 was detected by immunofluorescence staining using Laser scanning confocal microscope.Results： MGd1 could inhibit the cell proliferation and promote apoptosis of SGC7901 in dose-and time-dependent manners.The localization of MGd1-Ag was detected under confocal microscopy,and it showed that MGd1-Ag was mainly located in the membrane.Conclusion： MGd1 inhibits cell proliferation and promotes apoptosis of SGC7901 by banding with MGd1-Ag. |
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| Keywords: | Monoclonal antibody MGd1 Proliferation Apoptosis |
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