清胰汤对大鼠急性坏死性胰腺炎IL-6,IL-10及肠道通透性影响

目的：探讨清胰汤改善大鼠急性坏死性胰腺炎（acute necrotizing pancreatitis）ANP炎症反应及肠道通透性功能的治疗效果及机制。方法：将72只雄性SD大鼠随机分为3组,其中2组大鼠采用从胰腺被膜下多点缓慢均匀注入3.8%牛黄胆酸钠（0.5ml/100g）建立大鼠急性坏死性胰腺炎模型,再分为急性坏死性胰腺炎常规治疗组（A组）、清胰汤干预治疗组（B组）,其他24只大鼠为假手术组（S组）,每组再随机分为24h、48h、72h组。各组于12h后给于肠内营养,B组肠内营养后给于2次清胰汤2.5ml/100g,A组、S组给于同等剂量生理盐水。各组于建模后24h、48h、72h处死,腹腔动脉取血检测血清淀粉酶浓度、IL-6、IL-10、D-乳酸水平。结果：48h时点B组IL-10水平较A组高（P〈0.05）;72时点B组血清淀粉酶水平较A组低（P〈0.01）,IL-6水平较A组低（P〈0.01）,IL-10水平较A组高（P〈0.01）,D-乳酸水平较A组低（P〈0.01）。结论：清胰汤可以上调IL-10改善大鼠急性胰腺炎炎症反应从而降低肠道通透性。

Effect of Qingyitang on IL-6 IL-10 and Intestinal Permeability in Rats with Acute Necrotizing Pancreatitis

Objective： To investigate the effect and mechanism of Qingyitang on inflammation and gut dysfunction in rats with acute necrotizing pancreatitis （ANP）. Methods： 72 rats were randomly divided into 3 groups with 24 rats in each group. The rats of two groups were treated by injecting 0.5 ml/100g of 3.8 % sodium taurocholate beneath the pancreatic capsule to get the model of ANP. The 48 rats were divided into the ANP group （A group） and the ANP group treated with Qingyitang （B group）. The other 24 rats were sham operation group （S group）. The rats of each group were divided into 24h group, 48h group and 72h group. Eternal nutrition was given to rats in each group 12 hours after operation. Rats in group B were given Qingyitang （2.5 ml/100 g ） 2 times every day. Rats in other groups were treated with same volume of normal saline solution. Rats were sacrificed by abdominal aorta exsanguinations at 24th, 48th and 72nd hour after the operation. The blood samples were collected for analysis of serum amylase, IL-6, IL-10 and D-Lactate. Results： The serum IL-10 level of group B was higher than that of group A at 48th hour （P0.05）; The serum amylase, IL-6 and D-Lactate levels of group B were lower than that of group A （P0.01）, was higher than that of group A at 72nd hour （P0.01）. Conclusion： Qingyitang improves the IL-10 level and maintain the intestinal permeability.