| DLC-1基因在MCF-7人乳腺癌细胞系中低表达的机制探究 |
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| 引用本文： | 马小娟,覃君慧,信波,王雪莹,李颖,闫庆国,师建国,王瑞安. DLC-1基因在MCF-7人乳腺癌细胞系中低表达的机制探究[J]. 生物磁学, 2011, 0(7): 1231-1234 |
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| 作者姓名： | 马小娟 覃君慧 信波 王雪莹 李颖 闫庆国 师建国 王瑞安 |
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| 作者单位： | 第四军医大学西京医院病理科,陕西西安710032 |
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| 基金项目： | 国家自然科学基金（30870923）国家自然科学基金（30971535） |
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| 摘 要： | 目的：探究DLC-1基因在MCF-7人乳腺癌细胞系中低表达的机制。方法：应用甲基化特异性PCR（MSP）检测人乳腺癌细胞MCF-7的DLC-1基因甲基化状态,不同浓度的5-氮杂-2＇-脱氧胞嘧啶（5-Aza-CdR）处理人乳腺癌细胞MCF-7,RT-PCR及Real-time PCR定量检测用药前后细胞中DLC-1基因mRNA表达水平变化。结果：DLC-1基因启动子区CpG岛呈甲基化状态,经过5-Aza-CdR处理后,DLC-1基因启动子区呈去甲基化状态,并且其mRNA恢复表达。结论：抑癌基因DLC-1 CpG岛甲基化是导致该基因低表达的原因之一,5-Aza-CdR能逆转DLC-1基因甲基化状态。
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| 关 键 词： | DLC-1 启动子甲基化 乳腺癌 |
Study on Mechanism of Low Expression of Tumor Suppressor Gene DLC-1 in Human Breast Cancer Cell Line |
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| MA Xiao-juan,QIN Jun-hui,XIN Bo,WANG Xue-ying,LI Ying,YAN Qing-guo,SHI Jian-guo,WANG Rui-an. Study on Mechanism of Low Expression of Tumor Suppressor Gene DLC-1 in Human Breast Cancer Cell Line[J]. Biomagnetism, 2011, 0(7): 1231-1234 |
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| Authors: | MA Xiao-juan QIN Jun-hui XIN Bo WANG Xue-ying LI Ying YAN Qing-guo SHI Jian-guo WANG Rui-an |
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| Affiliation: | △(Department of Pathology,Xijing Hospital,The Fourth Military Medical University,Xi＇an 710032,China) |
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| Abstract: | Objective：To investigate the mechanism of low expression of tumor suppressor gene dlc-1 in human breast cancer cell line.Methods：Methylation-specific PCP（MSP） was used to examine the methylation status of DLC-1 promoter.MCF-7 cells were treated by different concentrations of 5-Aza-CdR.DLC-1 mRNA levels were examined by RT-PCR and Real-time PCR before and after treatment with 5-Aza-CdR.Results：There was methylation in DLC-1 promoter.The expression of DLC-1 mRNA in MCF-7 increased after treated by by 5-Aza-CdR.Conclusion：Methylation of DLC-1 promoter might be the main reason of the low expression of DLC-1 in MCF-7 human breast cancer cells,and the 5-Aza-CdR can inhibit this methylation. |
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| Keywords: | DLC-1 Promoter methylation Breast cancer |
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