| RP-HPLC法测定酸枣仁汤中芒果苷的含量 |
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| 引用本文： | 齐凤琴,李廷利,张博,张冰,郭冷秋,黄莉莉. RP-HPLC法测定酸枣仁汤中芒果苷的含量[J]. 生物磁学, 2011, 0(12): 2353-2355 |
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| 作者姓名： | 齐凤琴 李廷利 张博 张冰 郭冷秋 黄莉莉 |
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| 作者单位： | 黑龙江中医药大学,黑龙江哈尔滨150040 |
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| 基金项目： | 教育部2008年博士点基金（200802280001） |
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| 摘 要： | 目的：建立测定酸枣仁汤中芒果苷含量的反高效液相色谱分析方法。方法：色谱柱：WatersC18（250nm×4.6mm,5μm）;流动相：乙腈-0.1%磷酸水溶液,梯度洗脱;流速：1.0ml/min,检测波长：257nm。结果：芒果苷在0.316μg-1.264μg范围内与峰面积线性关系良好（r=0.9999）,平均加样回收率为97.64%,RSD为0.36%。结论：本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中芒果苷的含量测定方法。
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| 关 键 词： | 酸枣仁汤 芒果苷 RP-HPLC |
Determination of Mangiferin in Suanzaoren Decoction by RP-HPLC |
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| QI Feng-qin,LI Ting-li,ZHANG Bo,ZHANG Bing,GUO Leng-qiu,HUANG Li-li. Determination of Mangiferin in Suanzaoren Decoction by RP-HPLC[J]. Biomagnetism, 2011, 0(12): 2353-2355 |
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| Authors: | QI Feng-qin LI Ting-li ZHANG Bo ZHANG Bing GUO Leng-qiu HUANG Li-li |
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| Affiliation: | (Heilongjiang University of Traditional Chinese Medicine,Haerbin 150040,China) |
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| Abstract: | Objective：To establish a HPLC method for the determination of mangiferin in Suanzaoren Decoction.Methods：The determination was performed on WatersC18 column（250nm×4.6mm,5um）with a grient mobile phase composed of acetionitrile and 0.1% phosphoric acid.The detection wavelength was 257nm,and the flow rate was 1ml/min.Results：The Mangiferin showed good lin-earity（r=0.9999）in the range of 0.316ug-1.264ug,the average recovery was 97.64% with RSD 0.36%.Conclusion：The method is simple,accurate,reliable and suitable for determination of Mangiferin in Suanzaoren Decoction. |
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| Keywords: | Suanzaoren Decoction Mangiferin RP-HPLC |
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