成人急性髓性白血病SOCS-1基因及其甲基化的研究

目的：通过检测成人急性髓性白血病中SOCS．1基因表达水平及其甲基化水平，研究其在白血病发病中的作用。方法：运用甲基化特异性PCR（Methylation specificPCR，MSP）方法，对24例急性髓性白血病患者和4株白血病细胞株（Jurkat、Raji、U937、NALM17），进行SOCS-1基因甲基化水平的研究；同时运用Real—timePCR法定量分析SOCS—1基因表达水平。以10例健康人为正常对照组。结果：24例成人急性髓性白血病患者中，15例有SOCS-1基因甲基化（62．5％），而正常对照组无SOCS-1基因甲基化（0％），二者有显著差异（P〈0．05）；SOCS-1基因甲基化组与无SOCS-1基因甲基化组相比较，其SOCS—1基因相对表达量明显减少（P口0．05）；与患者临床病理特征相结合比较，发现SOCS-1基因的甲基化与患者年龄、性别和病程阶段无相关。4株白血病细胞株中，Jurkat和U937表现有SOCS—1甲基化（50％），Raji和NALM17无SOCS—1甲基化，前者SOCS-1基因表达量较后者也明显降低（P〈0．05）。结论：SOCS—1基因在成人急性髓性白血病中甲基化水平明显增高，且SOCS-1基因甲基化后表达水平受到抑制，提示SOCS-1基因及其甲基化在急性髓性白血病的发生发展中可能具有一定作用。

The Methylation of SOCS- 1 Gene in Adult Acute Myeloid Leukemia

Objective： To investigate the role of SOCS-1 （Suppressor of Cytokine Signalling-l） in tumorigenesis of adult acute myeloid leukemia （AML）by detecting the methylation state and expression of SOCS-1 gene. Methods： A total of 24 patients with AML, 10 healthy volunteers and 4 cell lines （Jurkat, Raji, U 937, NALM 17） were chosen. The methylation state of SOCS-1 in samples and cell lines were detected by using methylation-specific polymerase chain reation （MSP）, and the expression of SOCS-1 was detected by using Real-time PCR. Results： The aberrant methylation of SOCS-1 was 62.5 % （ 15/24 ）in primary patients with AML. In contrast, there was no aberrant methylation of SOCS-1 gene in normal samples （P〈0.05）. The expression of SOCS-1 in AML patients with aberrant methylation of SOCS-1 gene was lower than that in AML patients without aberrant methylation of SOCS-1 gene （P〈0.05）. There was no relation between the methylation of SOCS-1 gene and the clinicopathological data （such as sex, age and tumor stage）. Conclusion： The methylation of SOCS-1 gene in AML was higher than that in control group significantly （P〈0.05）, and aberrant methylation strongly correlates with reduced expression. SOCS-1 gene may play an important role in tumorigenesis of AML.