| 核酸提取方法进展 |
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| 引用本文： | 刘洪波,刘晓雷,罗小铭. 核酸提取方法进展[J]. 生物磁学, 2011, 0(16): 3187-3190 |
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| 作者姓名： | 刘洪波 刘晓雷 罗小铭 |
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| 作者单位： | [1]中山市疾病预防控制中心,广东中山528403 [2]华中科技大学同济医学院,湖北武汉430030 |
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| 摘 要： | 核酸提取是分子生物学的基本方法，也是核酸诊断中最关键的方法，它是下游诊断、分析和制备的前提。过去的核酸提取方法繁琐费时且有限。目前，核酸提取方法已经取得了较大进步。本文综述了核酸提取方法的进展情况，包括传统的基于溶液的抽提方法、现在常用的柱提取法、正兴起的多效生物分子抽提法和自动化抽提系统等。多效生物分子抽提法、自动抽提系统的微型化和完全自动化或者它们的组合是核酸提取技术未来发展的方向。
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| 关 键 词： | 核酸 分离和纯化 柱层析 实验室自动化 |
Developments of Nucleic Acid Extraction Method |
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| LIU Hong-bo,LIU Xiao-lei,LUO Xiao-ming. Developments of Nucleic Acid Extraction Method[J]. Biomagnetism, 2011, 0(16): 3187-3190 |
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| Authors: | LIU Hong-bo LIU Xiao-lei LUO Xiao-ming |
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| Affiliation: | 1 Zhongshan Center for Disease Control and Prevention, Zhongshan 528403, China; 2 Tongji Medical College, Huazhong University, of Science and Technology, Wuhan 430030,China) |
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| Abstract: | Extraction of nucleic acid （NA） is the basic method used in molecular biology and has now become the most critical step in NA-based diagnostic assays. It is the starting point for downstream diagnosis, analysis or preparation. In the past, the process of NA used to be complicated, time-consuming, labor-intensive, and limited in terms of overall throughput. Currently, it comes a long way over time. In this article, the developments of NA extraction methods are reviewed, including the conventional solution-based method, frequently-used solid-phase purification method, newly-rising all-in-one biomolecules extraction method and automated extraction system. The all-in-one biomolecules extraction method, miniaturized and fully-automated extraction system or a combination of them can be prospective developments in the future. |
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| Keywords: | Nucleic acid Isolation and purification Column chromatography Laboratory automation |
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