昆布（海带）对高脂血症大鼠血脂的调节作用和机制

目的：探讨昆布（海带）在实验性高脂血症大鼠中的降血脂作用和机制。方法：健康雌性Wistar大鼠40只,应用高脂饲料喂养方法建立高脂血症动物模型,海带粉饲料喂养干预治疗。生化法检测大鼠血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）水平。硫代巴比妥酸法和硝酸还原酶法分别检测脂质过氧化物丙二醛（MDA）和一氧化氮（NO）含量,黄嘌呤氧化酶法和化学比色法分别测定超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性。结果：经海带干预治疗后,动物血清TG、TC和LDL水平较模型组显著降低、HDL水平显著升高（P〈0.05）。治疗组动物血清和肝组织MDA和NO水平显著低于、而SOD和GSH-PX活性均显著高于模型组（P〈0.05）。结论：海带可能影响TG、TC、LDL和HDL等组分的代谢,通过增强抗氧化SOD和GSH-PX的活性,降低体内MDA和NO的水平,发挥调节血脂水平的作用。

Regulating Effect of Laminaria Japonica Powder on Serum Lipids ofHyperlipidemia in Rats

Objective： To investigate the effect and mechanism of Laminaria japonica（Laminariales,Phaeophyta） on serum lipid of hyperlipidemia in rats.Methods： Forty healthy female Wistar rats were used to establish hyperlipidemia models by feeding fat-rich for-age and the kelp powder was applied as raw materials for potential marine drugs.The levels of serum lipid including the triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL） were detected by biochemical assay.The concentrations of lipid perioxide malondialdehyde（MDA） and nitric oxide（NO） were respectively measured by thiobarbituric acid assay and nitrate reductase assay.The activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） were respectively deter-mined by xanthinoxidase assay and chemical colormetry.Results： After treated with kelp feeds,the serum levels of TG,TC and LDL de-creased while HDL increased significantly than those in model group rats（P0.05）.After treatment with kelp,the levels of MDA and NO in serum and hepatic tissue were lower than those in the model group rats（P0.05）,while the activities of SOD and GSH-PX were signif-icantly higher than those in the model group rats（P0.05）.Conclusion： L.japonica may interference the metabolism of TG,TC,LDL and HDL and by increasing the activities of SOD and GSH-PX to reduce the levels of MDA and NO to regulate the levels of serum lipids.