大鼠脑缺血再灌注后血清中血管内皮生长因子与神经元凋亡的研究

目的：通过检测SD大鼠脑缺血再灌注模型血清中血管内皮生长因子（VEGF）与神经元凋亡动态表迭变化的关系，以探讨两者之间的相关性。方法：将40只大鼠随机分为8组：对照组、假手术组和脑缺血30min再灌注12h组、1d组、3d组、5d组、7d组、及14d组，每组5只。采用ELISA双抗夹心法检测大鼠血清中血管内皮生长因子、原位细胞凋亡TUNEL法检测脑组织中的凋亡神经细胞数。结果：再灌注12h、1d、3d、5d、7d及14d大鼠血清VEGF表达和凋亡神经元百分比的变化均为负相关性（均为P〈0．05）。结论：在脑缺血再灌注大鼠模型中，缺血诱导使VEGF的表达发生变化，VEGF通过直接或间接的途径抑制神经元凋亡。

Relation Between Vascular Endothelial Growth Factor in Sera and Neuronal Apoptosis after Cerebral IschemicReperfusion in Rats

To investigate the interrelation ship between thedynamic changes of of VEGF and neuronal apoptosis after Cerebral Ischemic Reperfusion in SD Rats. Methods： A total of forty SD rats were randomly divided into eight different groups （n = 5）： controls group, sham-operation group, cerebral ischemia for 30 min and reperfusion for 12 hour（ I/R12h group）, 1 day（I/R 1 d group）, 3 day （I/R3d group）, 5 day（ I/R5d group）, 7 day（ I/R7d group）, and 14day（I/R14d group）. By using ELISA method the VEGF were measured, the apoptosis of neurons was examined by TUNEL. Results： The expression of VEGF has a negative correlation （P〈0.05） with neurons apoptosis reperfusion for 12hour, 1 day, 3 day, 5day, 7day and 14day after Cerebral Ischemic Reperfusion. Conclusions： VEGF mediated by cerebral ischemia inhibits the apoptosis of neurons in rats after cerebral ischemic reperfusion.