| Fra-1对大鼠C6胶质瘤细胞侵袭转移能力的影响 |
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| 引用本文： | 田刚,王晓娟,雷宁,曾庚乐,樊明新,徐英,杨江河,卢宁,李华. Fra-1对大鼠C6胶质瘤细胞侵袭转移能力的影响[J]. 生物磁学, 2011, 0(16): 3038-3040 |
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| 作者姓名： | 田刚 王晓娟 雷宁 曾庚乐 樊明新 徐英 杨江河 卢宁 李华 |
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| 作者单位： | [1]兰州军区乌鲁木齐总医院神经外科,新疆乌鲁木齐830000 [2]解放军第23医院临床部药械科,新疆乌鲁木齐830000 [3]成都理工大学材料与化学化工学院化工与制药系,四川成都610059 [4]兰州军区乌鲁木齐总医院肿瘤科,新疆乌鲁木齐830000 |
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| 摘 要： | 目的：观察Fra-1对C6胶质瘤侵袭转移能力的影响。方法：以大鼠C6胶质瘤细胞为研究对象，Fra-1siRNA通过脂质体转染C6，realtimeRT-PCR和westernblot法检测C6细胞中Fra-1的表达、Elisa法检测细胞培养上清中MMP一9的含量，Transwell小室观察C6细胞的转移侵袭能力。结果：干扰Fra-1后能降低C6细胞中Fra-1的表达，显著降低C6细胞中MMP-9的含量，降低C6细胞的转移侵袭能力。结论：干扰Fra-1能抑制C6细胞的转移侵袭。
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| 关 键 词： | Fra-1 基质金属蛋白酶 C6 |
Effects of Fra-1 on Invasive Ability of C6 Glioma Cells |
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| Affiliation: | TIAN Gang, WANG Xiao-juan , LEI Ning , ZENG Qing-le, FAN Ming-xin , XU Ying , YANG Jiang-he , LU Ning , LI Hue (1 Department of neurosurgery of Urumchi General Hospital in Lanzhou Military Area Command, Urumchi 830000; 2 Department of Plant Protection Machine, The 23th hospital of Chinese People＇s Liberation Army, Urumehi 830000; 3 Department of Chemical ＆ Pharmaceutical Engineering, College of Material and Chemistry ＆ Chemical Engineering, Chengdu University of Technology. Chengdu 610059;4 Department omedical onaology of Urumchi General Hospital in Lanzhou Militaty Area Command, Ummchi, 830000) |
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| Abstract: | Objective： To study the effect ofFra-lon invasive ability of C6 cells. Methods： C6 cells were divide into blank group, control group, Fra-lsiRNA groups. The effects of Fra-I siRNA on C6 cells invasive ability in vitro was measured by Boyden chamber invasive models. Contents of Fra-1 protein and mRNA were detected by western blot and realtime RT-PCR, respectively. Contents of MMP-9 were measured by ELISA methods. Results： In siRNA group, contents of Fra-1 and MMP-9 were decreased markedly, the invasive ability was decreased markedly, too. Conclusion： Fra-1 siRNA could decrease the invasive ability of C6 cell. |
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| Keywords: | Fra-1 Matrix metalloproteinase C6 |
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