腹腔注射法制备一氧化碳中毒迟发性脑病大鼠模型

目的：建立可靠的急性一氧化碳（CO）中毒迟发性脑病的动物模型。方法：雄性SD大鼠,分次腹腔注射CO染毒制备模型,动态监测尾血碳氧血红蛋白（HbCO）浓度;Morris水迷宫检测大鼠1-5w逃避潜伏期;尼氏染色及TUNEL原位末端凋亡染色检测大脑皮质及海马细胞损伤及凋亡。结果：染毒后,大鼠出现典型的CO重度中毒症状,体内血液HbCO浓度迅速升高,使用分次腹腔注射法,大鼠可维持长时间（〉12h）高HbCO状态（HbCO〉48%）;中毒组大鼠水迷宫检测认知功能较对照组下降,病理学检查显示大鼠出现脑细胞损伤、凋亡明显。结论：本研究建立了一种较为符合迟发性脑病临床特征的动物模型,具有简单、可靠、重复性好的特点,为深入研究急性CO中毒致迟发性脑损伤的机制提供可靠基础。

Preparation of carbon monoxide poisoning delayed encephalopathy rat model by intraperitoneal injection

Objective：To establish a convenient and reliable delayed neuropsychologic sequelae rat model with acute carbon monoxide（CO） poisoning.Methods：Male SD rats were injected CO repeatedly,then dynamically monitored the carboxyhemoglobin（HbCO） level in blood for ascertaining the administration dose and process;Cognitive ability was measured by the escape latency of searching the objective in the Morris watermaze;Neuronal injury were detected by Nissl stain,TUNEL stain was used to assess apoptosis.Results：The HbCO levels rised rapidly,and kept at higher level（48%） for long time（12h） by the method of repeated injection of CO ip;The cognitive ability in CO group was lower than that of the control group;The pathological examination revealed apparent neuronal degeneration and necrosis in the cerebral in CO poisoned rats.Conclusion：CO peritoneum injection was aeffective,convenient,and reli-able,which offered reliable scientific basis for further exploring the pathogenesis of DNS by CO.