Chromosome sorting of primates: Isolation and identification of Y chromosome in green monkeys |
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Authors: | Yuichi Murayama Hiroaki Shibata Takeyuki Nagashima Miki Takuwa Atsuo Noguchi Keiji Terao |
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Institution: | (1) Institute of Basic Medical Sciences, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(2) Tsukuba Primate Center for Medical Science, The National Institute of Health, 305 Tsukuba, Ibaraki, Japan |
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Abstract: | Single laser flow cytometry was applied to the karyotype analysis of green monkeys. Clear sex difference in flow karyotype
was recognized in this monkey, because Y chromosome could be identified as a single peak in the histogram of male specimens.
We could isolate Y chromosome of this species by the use of a cell sorter, and demonstrate by polymerase chain reaction that
the sorted-out chromosomes contained the Y chromosome specific nucleotide sequence (SRY). This chromosome sorting technique provides a powerful strategy for constructing the DNA library specific to Y chromosome
in this species. |
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Keywords: | Flow karyotype Chromosome isolation Y chromosome SRY gene DYZ1 locus Green monkeys |
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