Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples |
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| Authors: | Andrea Vivian Alvarez-Oxiley Noelita Melo de Sousa Jean-Luc Hornick Kamal Touati Gysbert C van der Weijden Marcel AM Taverne Otto Szenci Jean-François Beckers |
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| Affiliation: | (1) Laboratory of Endocrinology and Animal Reproduction, Faculty of Veterinary Medicine, University of Liege, Belgium;(2) Nutrition of Large Animals, Faculty of Veterinary Medicine, University of Liege, Belgium;(3) Clinic of Large Animals, Faculty of Veterinary Medicine, University of Liege, Belgium;(4) Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht, the Netherlands;(5) Clinic for Large Animals, Faculty of Veterinary Science, Szent Istvan University, Budapest, Hungary |
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| Abstract: | Background The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions. |
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