Investigating the effects of vitreous humour (crude extract) on growth and differentiation of rat mesenchymal stem cells (rMSCs) and human NTERA2 cells |
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Authors: | A J Bagherpoor A R Bahrami M M Matin N Mahdavi-Shahri M A Edalatmanesh |
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Institution: | 1.Cellular and Molecular Biology Research Group, Institute of Biotechnology,Ferdowsi University of Mashhad,Mashhad,Iran;2.Department of Biology, Faculty of Sciences,Ferdowsi University of Mashhad,Mashhad,Iran |
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Abstract: | Two main characteristics of all types of stem cells are their potency for differentiation and self renewal capacity. There
is a lot of interest to find the conditions and factors, which govern these behaviours of stem cells. It is very well documented
that retinoic acid (RA) reduces growth rate by induction of cell differentiation in certain conditions and cell lines. On
the other hand, hyaluronic acid (HA) is known for its growth induction on cultured cells. A natural source of HA, rabbit vitreous
humour (VH), was previously shown to promote wound repair in model animals. In search for its possible mechanisms, VH extract
was tested on the cultured mesenchymal stem cells and NTERA2 as human embryonal carcinoma cells in the presence of RA. Changes
in some cellular and molecular markers (A2B5, 0ct4, Sox2) showed that VH and possibly HA interfere with differentiating effects
of RA. Therefore, this reagent may affect cell proliferation and tissue regeneration by inhibition of cell differentiation. |
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