Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation |
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Authors: | Chang Duk Kang Se Jong Han Seung Phill Choi Sang Jun Sim |
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Institution: | (1) Nano Optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea;(2) Polar BioCenter, Korea Polar Research Institute, KORDI, Incheon, 406-840, Korea; |
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Abstract: | A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture
of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding
strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light
depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity
of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the
light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day.
The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of
valuable Haematococcus astaxanthin. |
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Keywords: | |
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