Comparative molecular analysis of <Emphasis Type="Italic">Fusarium solani</Emphasis> isolates by RFLP and RAPD |
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| Authors: | V K Gupta P K Jain A K Misra R Gaur R K Gaur |
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| Institution: | 1.Department of Biotechnology,MITS University,Laxmangarh,India;2.Central Institute for Subtropical Horticulture,Lucknow,India;3.Dr. R.M.L. Avadh University,Faizabad,India |
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| Abstract: | Fusarium solani is an important pathogen causing wilt disease of guava in India. In this work, we analyzed seven representative isolates
of F. solani, collected from different places of India, by restriction fragment length polymorphism (RPLP) using HindIII or DraI restriction endonucleases and random amplified polymorphic DNA (RAPD). Pattern of restriction enzyme revealed a similar
restriction cut type cluster in the isolate namely, Allahabad (isolate-3), Faizabad (isolate-4), Unnao (isolate-5) and Lucknow
(isolate-6) region, while other cluster was consist of isolate from Ranchi (isolate-2) and Ludhiana (isolate-7). Slightly
variable results were obtained when 10 randomly amplified polymorphic DNA markers (OPA01–OPA10) tested in the genome of Fusarium solani and grouped on basis of obtained allelic data. RAPD fingerprinting showed a higher variability than RFLP, and each isolate
had a unique electrophoretic pattern with five of the ten primers used. Our results show that RAPD much efficient to distinguish
between all F. solani isolate tested. |
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