Gene transfer by electroporation in betulaceae protoplasts: Alnus incana

The Escherichia coli -glucuronidase gene (GUS) was introduced into Alnus incana (L.) Moench protoplasts by electroporation. Level of GUS transient gene expression was increased by increasing DNA concentrations of pBI 221 plasmid and was affected by the amplitude and duration of the applied electric pulse as well as by the presence of polyethylene glycol (PEG) in the electroporation medium. An optimal level of GUS activity was obtained after electroporation with a capacitive discharge of 500 V/cm and 71 ms-duration. This transformation procedure is simple and efficient. These results motivated us to investigate this method as a possible way of achieving the stable transformation of actinorhizal alder.Abbreviations CaMV cauliflower mosaic virus - CPW salts, Cocking-Power-White salts - kb kilobase(s) - MU 4-methyl umbelliferyl - MUG 4-methyl umbelliferyl glucuronide - F microfarad(s) - NOS nopaline synthase