Modification of an algal culture medium for sustained growth of a saxitoxin-producing isolate of Alexandrium minutum |
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Authors: | Elizabeth W Maas Jurgen Thiele Caryn Thompson Rebecca M Latter Heather JL Brooks |
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Institution: | (1) Department of Microbiology, University of Otago, Dunedin, New Zealand;(2) Department of Mathematics and Statistics, University of Otago, Dunedin, New Zealand |
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Abstract: | In order to study saxitoxin (STX) production bymicro-algae in the laboratory, a defined algal culture medium which supports
optimum growth over a longtime-period is a requirement. In the development of such a medium, a number of modifications were
made to a standard algal culture medium (GP) and growth of a STX-producing isolate of Alexandrium minutum in the different formulations was assessed by measuring maximum cell densities and mean generation times (MGT). All experiments
were carried out under controlled conditions in an aerobic atmosphere with increased CO2. Whilst maximum cell densities in the different modifications were similar, the MGT was significantly shortened by the addition
of Tris buffer and the trace metals strontium, selenium and molybdenum. Replacement of natural with artificial seawater and
removal of soil extract did not adversely affect algal growth. Five of the six media formulations supported the growth of
A. minutumover a 9-month period.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Alexandrium minutum growth medium micro-algae nutrients |
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