Use of high pressure liquid chromatography for analysis of deoxyribonucleotides in the epidermis |
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| Authors: | Ryohei Ogura Tadasu Matsuzaki Shuji Kumano Abdulwahid H Daoud Clark A Griffin John M Knox |
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| Institution: | 1. Department of Medical Biochemistry, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, Japan 830;2. Department of Biochemistry, The University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, Houston, Texas 77035 USA;3. Department of Dermatolog, Baylor College of Medicine, Houston, Texas 77030, USA |
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| Abstract: | An analytical method for free deoxyribonucleotide (nucleoside monophosphate) in the epidermis is presented. Free nucleotides were extracted from tissue using a methylal ethanol mixture. The deoxyribonucleotides were separated using DEAE-cellulose and DBAE-cellulose. The analysis of free deoxyribonucleotides was carried out by high pressure liquid chromatography on a column of Lichrosorb-NH2 with a single buffer of potassium phosphate. The elution order of nucleotides was CMP, AMP, UMP, IMP, GMP, and XMP. This procedure employing high pressure liquid chromatography for the detection of deoxyribonucleotides in the epidermis makes it possible to elucidate the biological characteristics and significance of DNA metabolism in normal epidermis and changes that occur in pathological conditions. |
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