Affinity chromatography of lactate dehydrogenase on immobilized nucleotides |
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| Authors: | C R Lowe and P D G Dean |
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| Institution: | Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K. |
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| Abstract: | The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immobilized NAD(+) was investigated. The interaction of both lactate dehydrogenase isoenzymes with immobilized pyridine and adenine nucleotides and their derivatives were measured. The effects of these parameters on the interaction of lactate dehydrogenase with immobilized nucleotides were correlated with the known kinetic and molecular properties of the enzymes in free solution. |
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