Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birds |
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| Authors: | Erika S Tavares Allan J Baker |
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| Affiliation: | (1) Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Canada;(2) Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Canada |
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| Abstract: | Background DNA barcoding of life using a standardized COI sequence was proposed as a species identification system, and as a method for detecting putative new species. Previous tests in birds showed that individuals can be correctly assigned to species in ~94% of the cases and suggested a threshold of 10× mean intraspecific difference to detect potential new species. However, these tests were criticized because they were based on a single maternally inherited gene rather than multiple nuclear genes, did not compare phylogenetically identified sister species, and thus likely overestimated the efficacy of DNA barcodes in identifying species. |
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