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| 口蹄疫病毒O/QYYS/s/06株感染性克隆的构建 | |
| 引用本文： | 卢受昇,赵启祖,刘湘涛,孙彦伟,任涛,张桂红,亓文宝,查云峰,孔令辰,张翰,樊惠英,廖明.口蹄疫病毒O/QYYS/s/06株感染性克隆的构建[J].生物工程学报,2009,25(7):982-986. |
| 作者姓名： | 卢受昇 赵启祖 刘湘涛 孙彦伟 任涛 张桂红 亓文宝 查云峰 孔令辰 张翰 樊惠英 廖明 |
| 作者单位： | 1. 华南农业大学兽医学院,广州,510642;广东省动物防疫监督总所,广州,510230 2. 中国兽医药品监察所,北京,100081 3. 中国农业科学院兰州兽医研究所,兰州,730046 4. 广东省动物防疫监督总所,广州,510230 5. 华南农业大学兽医学院,广州,510642 |
| 基金项目： | 国家自然科学基金(No.30800826)资助~~ |
| 摘 要： | 本研究在完成FMDVO/QYYS/s/06株全基因组序列测定的基础上,分3段对全基因组进行克隆,其后将各片段克隆到载体P43中,从而获得携带O/QYYS/s/06株基因组全长cDNA的重组质粒P43C。将重组质粒P43C与表达RNA聚合酶的质粒T7共转染BHK-21细胞,48h后收获培养液接种2~3d乳鼠,取经乳鼠传代后的第4代病毒液,经反向间接血凝、中和试验和测序等方法证明拯救的病毒为O型FMDV。以上结果表明,O/QYYS/s/06株全长cDNA分子克隆的构建成功。 |
| 关 键 词： | 口蹄疫病毒 O/QYYS/s/06株 全长cDNA |
| 收稿时间： | 1/4/2009 12:00:00 AM |
Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06 |
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| Shousheng Lu,Qizu Zhao,Xiangtao Liu,Yanwei Sun,Tao Ren,Guihong Zhang,Wenbao Qi,Yunfeng Zh,Lingchen Kong,Han Zhang,Huiying Fan and Ming Liao.Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06[J].Chinese Journal of Biotechnology,2009,25(7):982-986. | |
| Authors: | Shousheng Lu Qizu Zhao Xiangtao Liu Yanwei Sun Tao Ren Guihong Zhang Wenbao Qi Yunfeng Zh Lingchen Kong Han Zhang Huiying Fan and Ming Liao |
| Institution: | College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision, Guangzhou 510230, China;China Institute of Veterinary Drug Control, Beijing 100081, China;Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision, Guangzhou 510230, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision, Guangzhou 510230, China;Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision, Guangzhou 510230, China;Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision, Guangzhou 510230, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
| Abstract: | After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four g... |
| Keywords: | foot-and-mouth disease virus (FMDV) O/QYYS/s/06 strain full-length cDNA |
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