杜仲次生木质部分化过程中的细胞编程死亡 |
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引用本文: | 曹静,贺新强,王雅清,苏都莫日根,崔克明.杜仲次生木质部分化过程中的细胞编程死亡[J].Journal of Integrative Plant Biology,2003,45(12):1465-1474. |
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作者姓名: | 曹静 贺新强 王雅清 苏都莫日根 崔克明 |
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作者单位: | 北京大学生命科学学院,北京,100871 |
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基金项目: | 国家重点基础研究发展计划(973计划),国家自然科学基金 |
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摘 要: | 通过电子显微镜观察、DNA断裂检测及类似半胱氨酸蛋白酶(caspase-like proteases,CLPs)降解检测等技术,对杜仲(Eucommia ulmoides Oliv.)次生木质部分化过程的细胞编程死亡进行了研究.分化中的次生木质部细胞总DNA凝胶电泳检测到DNA ladder,并通过TUNEL检测进一步确定了DNA被降解.Western blot结果表明;caspase-8和caspase-3状蛋白酶(caspase-8-和caspase-3-like proteases,CLPs)及多聚ADP-核糖聚合酶(poly(ADP-ribose)polymerase,PARP)在次生木质部分化过程中被降解.这些研究结果表明,杜仲次生木质部的细胞分化是一个典型的编程性死亡(Programmed cell death,PCD)过程,CLPs可能参与了此过程.
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关 键 词: | Caspase状蛋白酶 DNA片段化 杜仲 多聚ADP-核糖聚合酶(PARP) 细胞编程死亡 次生木质部分化 |
Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides |
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Authors: | CAO Jing HE Xin-Qiang WANG Ya-Qing Sodmergen CUI Ke-Ming |
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Abstract: | Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gel electrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-end labeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-like proteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation. The results thus indicated that secondary xylem differentiation in E. ulmoides was a typical process of PCD and the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation. |
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Keywords: | caspase-like protease DNA fragmentation Eucommia ulmoides poly (ADP-ribose) polymerase programmed cell death secondary xylem differentiation |
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