首页 | 本学科首页   官方微博 | 高级检索  
     


Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning
Authors:Wåhlander Asa  Arrigoni Giorgio  Wårell Kristofer  Levander Fredrik  Palmgren Ronnie  Maloisel Jean-Luc  Busson Philippe  James Peter
Affiliation:Department of Protein Technology, Lund University, Sweden.
Abstract:We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号