Salicylic acid changes the properties of extracellular peroxidase activity secreted from wounded wheat (Triticum aestivum L.) roots |
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Authors: | Minibayeva F Mika A Lüthje S |
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Institution: | (1) Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, RU;(2) Institute of General Botany, University of Hamburg, Hamburg, DE |
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Abstract: | Summary. Wheat (Triticum aestivum L.) roots released proteins showing peroxidase activity in the apoplastic solution in response to wound stress. Preincubation
of excised roots with 1 mM salicylic acid at pH 7.0 enhanced the guaiacol peroxidase activity of the extracellular solution
(so-called extracellular peroxidase). The soluble enzymes were partially purified by precipitation with ammonium sulfate followed
by size exclusion and ion exchange chromatography. Despite an increase in the total activity of secreted peroxidase induced
by pretreatment of excised roots with salicylic acid, the specific activity of the partially purified protein was significantly
lower compared to that of the control. Purification of the corresponding proteins by ion exchange chromatography indicates
that several isoforms of peroxidase occurred in both control and salicylic acid-treated samples. The activities of the extracellular
peroxidases secreted by the salicylic acid-treated roots responded differently to calcium and lectins compared with those
from untreated roots. Taken together, our data suggest that salicylic acid changes the isoforms of peroxidase secreted by
wounded wheat roots.
Received June 10, 2002; accepted September 24, 2002; published online May 21, 2003
RID="*"
ID="*" Correspondence and reprints: Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, Kazan
420111, Russia. |
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Keywords: | : Triticum aestivum Wheat root Wound stress Peroxidase Salicylic acid |
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