Multiplexed detection of foodborne pathogens based on magnetic particles |
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| Institution: | 1. Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain;2. Applied Enzyme Technology Ltd., Gwent Group Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK;1. Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh 11533, Saudi Arabia;2. Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Amman 11942, Jordan;3. King Faisal Specialist Hospital and Research Center, Zahrawi Street, Al Maather, Riyadh 12713, Saudi Arabia;1. Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain;2. Laboratory of Cellular Immunology, Institute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, Bellaterra, Spain;1. Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain;2. Applied Enzyme Technology Ltd., Gwent Group Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK;3. Grup de Microbiologia Molecular, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain;1. MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China;2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China;3. State Key Lab of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Science, Beijing, China;4. Modern Precision Agriculture System Integration Research Key Laboratory of Ministry of Education, China Agricultural University, Beijing, China;1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran;2. Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | This paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes. End-point and real-time PCR, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of magnetic particles in these approaches. Although the immunomagnetic separation for capturing single bacteria from contaminating microflora and interfering food components has demonstrated to improve the performance on these approaches, the integration of magnetic particles for multiplex detection of bacteria is still in a preliminary stage and requires further studies. |
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