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Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination

Affiliation:	1. The Forsyth Institute, Cambridge, MA, United states;2. Cummings School of Veterinary Medicine at Tufts, Grafton, MA, United states;3. Department of Animal Resources Science at Dankook University, Cheonan, South Korea;1. Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA 70112, USA;2. Department of Pediatrics, Tulane University School of Medicine, New Orleans, LA, USA;1. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), A.P.70228, Ciudad Universitaria, México, DF 04510, Mexico;2. Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (UNAM), A.P.70228, Ciudad Universitaria, México, DF 04510, Mexico;3. Programa de Doctorado en Ciencias Bioquímicas, Universidad Nacional Autónoma de México (UNAM), A.P.70228, Ciudad Universitaria, México, DF 04510, Mexico;4. Servicio de Patología Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Delegación Tlalpan, Mexico;1. Instituto de Estudios en Inmunología y Fisiopatología (IIFP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;2. Laboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT La Plata CONICET, La Plata, Argentina;1. Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy;2. Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome 00185 Rome, Italy;3. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA;4. Division of Virology, Department of Microbiology and Immunology, and International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Japan;5. Infection-Induced Host Responses Project, Exploratory Research for Advanced Technology, Saitama 332-0012, Japan;1. Equipe de Biovectorologie, Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France;2. Inserm UMR_S 1121, 11 rue Humann, 67085 Strasbourg, France

Abstract:	The development of vaccine approaches that induce mucosal and systemic immune responses is critical for the effective prevention of several infections. Here, we report on the use of the abundant human oral commensal bacterium Streptococcus mitis as a delivery vehicle for mucosal immunization. Using homologous recombination we generated a stable rS. mitis expressing a Mycobacterium tuberculosis protein (Ag85b). Oral administration of rS. mitis in gnotobiotic piglets resulted in efficient oral colonization and production of oral and systemic anti-Ag85b specific IgA and IgG antibodies. These results support that the commensal S. mitis is potentially a useful vector for mucosal vaccination.

Keywords:	Ag85b Vaccine vector Oral immunization Mucosal vaccine Gnotobiotic piglets
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