A proteolytic enzyme, trypsin, for immunofluorescence observation of microtubules in algal cells |
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| Authors: | Hirotumi Aruga Taizo Motomura Terunobu Ichimura |
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| Institution: | Institute of Algological Research, Hokkaido University, Muroran 051, Japan |
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| Abstract: | The usefulness of a proteolytic enzyme, trypsin, in immunofluorescence microscopy was confirmed in algal ceils. Flagella of Umspora penicilliformis zoo-spores were visible using an anti-β tubulin antibody after trypsin treatment, and the cortical microtubules of vegetative cells could also be clearly detected. Interestingly, centrioles that were not detected in the control observation appeared in gametophyte cells of Acrosiphonia duriuscula and Monostroma angicava using the trypsin treatment. |
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| Keywords: | algal cells centrioles immunofluorescence microscopy microtubules trypsin |
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