Water and monovalent ions in the minor groove of B-DNA oligonucleotides as seen by NMR |
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Authors: | Halle B Denisov V P |
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Affiliation: | Physical Chemistry 2, Lund University, P. O. Box 124, S-22100 Lund, Sweden. bertil.halle@fkem2.lth.se |
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Abstract: | During the past 8 years, two complementary nmr techniques-magnetic relaxation dispersion and nuclear Overhauser effect spectroscopy-have been applied extensively to the study of water and monovalent ions in the minor groove of B-DNA oligonucleotides in solution. In this review, the possibilities and limitations of the two methods are outlined, with emphasis on the interpretational steps whereby molecular-level information is extracted from the primary data. The results on sequence-dependent hydration and ion-DNA interactions obtained so far by these methods is summarized and critically assessed. The nmr results are also compared with structural data from x-ray crystallography. |
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