Flow cytometric competitive binding assay for determination of actinomycin-D concentrations |
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| Authors: | G C Saunders J C Martin J H Jett A Perkins |
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| Institution: | Cell Biology Group, Los Alamos National Laboratory, New Mexico 87545. |
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| Abstract: | A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 microns diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin-D concentration. |
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