Pancreatic glucagon in human foetal stomach |
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| Authors: | A M J Buchan M G Bryant B A Stein M Gregor M A Ghatei J F Morris S R Bloom J M Polak |
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| Institution: | (1) Departments of Histochemistry and Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W12 OHS London, England;(2) Department of Human Anatomy, South Parks Road, Oxford, England;(3) Present address: Department of Anatomy, Medical School, University of Witwatersrand, Hospital Street, 2001 Johannesburg, South Africa |
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| Abstract: | Summary A combination of immunocytochemistry at light and electron microscopic levels, direct radioimmunoassay and measurement after gel chromatography have been used to identify and characterise a glucagon-like peptide detected in human foetal stomach. Immunocytochemistry, with region specific antisera, demonstrated that the glucagon-containing cells were indistinguishable from pancreatic A cells. Radioimmunoassay of tissue extracts confirmed the presence of significant quantities of glucagon, mean 21 pmol/g wet weight (range 14–29) in 16–26 week old foetuses, increasing to 41 pmol/g wet weight (range 31–52) in 33–30 week old foetuses and after gel chromatography the peptide was found to elute at the same position as standard porcine glucagon. It is apparent, therefore, that the human foetal fundus contains significant quantities of true pancreatic-type glucagon.DeccasedPreliminary details of this work were presented at the Society for Endocrinology Meeting, London, November 1981 |
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