细菌人工染色体文库的构建及应用

细菌人工染色体（BAC）是第二代大片段DNA的克隆载体系统，具有容量大、嵌合率低、遗传特性稳定、转化效率高、插入片段易回收、操作简便等优点，因而被广泛应用于基因组较大的真核生物基因组研究中，并发挥着前所未有的重要作用。本文综述了BAC的发展，利用此载体构建基因组文库的程序和鉴定方法，及其在物理图谱构建、图位克隆、基因组测序、转基因技术等研究中的应用。

Construction and Application of Bacterial Artificial Chromosome Library

Bacterial artificial chromosome(BAC)is the secondary vector system for large DNA fragment cloning.Being capable of inserting large fragments,producing few cherisms,maintaining the facility and stability of inserted DNA and being easy in library construction,BAC is being widely used and playing important role in the research of larger eukaryote genomes.This paper presents a brief introduction of the development of BAC,the way of construction and identification of BAC library,and its applications in genomic physical mapping,genes map-based cloning,genomic sequencing and gene structure and function study.