H-2 antigens as genetic markers: A two-dimensional gel electrophoretic study |
| |
Authors: | Janet A. Sawicki Charles J. Epstein |
| |
Affiliation: | (1) Departments of Pediatrics and Biochemistry and Biophysics and Howard Hughes Medical Institute, Laboratories University of California San Francisco, California |
| |
Abstract: | Direct immunoprecipitation and two-dimensional (2D) gel electrophoresis have been used to identify and characterize genetic variation of theH-2K andH-2D regions. Using inbred strains of mice and alloantisera, haplotype-specific polypeptides were defined for five differentH-2 haplotypes. Specific immunoprecipitates prepared from strains of different haplotypes were applied to 2D gels in pairwise combinations to determine whether peptides specific to one haplotype can be distinguished from peptides specific to another. Those haplotype-specific peptides that migrate to unique positions on 2D gels with respect to the positions occupied by haplotype-specific peptides of another haplotype are useful as biochemical genetic markers. Cross-reactivity amongK- andD-region antigens of different haplotypes was identified on 2D gels and found to correlate well with existing data based on serological cross-reactivity. An anti-mouse 2-microglobulin serum was found to be a useful general reagent for immunoprecipitating haplotype-specific H-2 antigens to permit their visualization on 2D gels.Abbrevations used in this paper NP-40 nonidet P-40 - 2D two-dimensional - SDS sodium dodecyl sulfate - IEF isoelectric focusing |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |