40 S subunits from rat liver ribosomes contain two codon-dependent sites for transfer RNA |
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Authors: | A Comis S B Easterbrook-Smith |
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Affiliation: | 1. B.P. Konstantinov Nuclear Physics Institute, USSR Academy of Sciences, Gatchina, Leningrad district 188350, USSR;2. Central Institute of Molecular Biology, Department of Cell Physiology, GDR Academy of Sciences, 1115 Berlin-Buch, GDR |
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Abstract: | 40 S subunits from rat liver ribosomes are able to bind, after heat activation, two molecules of either Phe-tRNAPhe, Ac-Phe-tRNAPhe or deacylated tRNAPhe. Addition of 60 S subunits to the quaternary complex 40 S.poly(U).(Phe-tRNAPhe)2 results in quantitative formation of (Phe)2-tRNAPhe. This indicates that the two binding sites for tRNA on 40 S subunits should be considered as the constituent of P and A sites of 80 S ribosomes. |
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